Memorably depressing animated film you’ve seen?

What's the saddest animated film you've ever watched? I mean genuine sadness, not cheap melodrama and sensationalism. Open to both English-language films and foreign movies.

I don't have one that comes to mind, though there are specific scenes I remember that made me sad, like a certain scene in Toy Story 3 the incinerator scene. And there are so many genuinely touching scenes in the movie UP that reminds me of my grandparents and of promises people make and dreams never realized.

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